Development of technology for collecting and harvesting the herb of Linum pallescens and Linum heterosepalum

Development of an optimal technology for the collection and harvesting of Linum pallescens Bunge. and Linum heterosepalum Regel. herb is aimed at ensuring the preservation of biological activity, raw material quality, high technological efficiency and environmental sustainability of the processes. The implementation of the “Quality by Design” (QbD) concept in the development of technological processes makes it possible to systematically account for risks influencing critical quality attributes and to establish conditions for their optimization. The aim of the present study is to develop an optimal technology for the collection and harvesting of Linum pallescens and Linum heterosepalum herb to ensure quality based on the QbD concept. The plant specimens were identified by employees of the RSE REM «Institute of Botany and Phytointroduction». Within the framework of the QbD concept, statistical analysis with Minitab software was performed to establish the design space for the drying and harvesting processes of plant raw materials from both Linum species. The integration of the QbD concept into the process of developing the technology for the collection and harvesting of Linum pallescens and Linum heterosepalum herb demonstrated high efficiency. The results confirm the potential of applying QbD to improve quality, efficiency and control at all stages of medicinal plant raw material harvesting.

1. Pavlov N. V. (ed.). Flora Kazahstana: v 6 t., t. 6 [Flora of Kazakhstan: in 6 vols., vol. 6]. Alma-Ata: Izd-vo Akademii nauk Kazahskoj SSR, 462 p., 1961.

2. Koçak, M. Z. “Phenolic Compounds, Fatty Acid Composition and Antioxidant Activities of Some Flaxseed (Linum usitatissimum L.) Varieties: A Comprehensive Analysis.” Processes 12 (2024): 689. https://doi.org/10.3390/pr12040689

3. Wang, S., Z. S. Zhang, T. F. Zhang, and D. W. Xue. “Extraction and Characterization of Flaxseed Oil Obtained with Subcritical n-Butane.” Journal of Oleo Science 69 (2020): 1011–20.

4. Nedelcheva, R., Y. Zarev, R. Mihaylova, E. Kozuharova, G. Momekov, and I. Ionkova. “Arylnaphthalene Lignans with a Focus on Linum Species: A Review on Phytochemical, Biotechnological and Pharmacological Potential.” Pharmacia 71 (2024): e117690. https://doi.org/10.3897/pharmacia.71.e117690

5. Parikh, M., and G. N. Pierce. “Dietary Flaxseed: What We Know and Don’t Know about Its Effects on Cardiovascular Disease.” Canadian Journal of Physiology and Pharmacology 97 (2019): 75–81.

6. Buckner, A. L., C. A. Buckner, S. Montaut, and R. M. Lafrenie “Treatment with Flaxseed Oil Induces Apoptosis in Cultured Malignant Cells.” Heliyon 5 (2019): e02251.

7. Ansari, R., M. M. Zarshenas, and A. H. Dadbakhsh “A Review on Pharmacological and Clinical Aspects of Linum usitatissimum L.” Current Drug Discovery Technologies 16, no. 2 (2019): https://doi.org/10.2174/1570163815666180521101136148–58. PMID: 29779483.

8. World Health Organization. WHO Guidelines on Good Agricultural and Collection Practices (GACP) for Medicinal Plants. Geneva: World Health Organization, 2003.

9. Politis, S. N., P. Colombo, G. Colombo, D. M. Rekkas “Design of Experiments (DoE) in Pharmaceutical Development.” Drug Development and Industrial Pharmacy 43, no. 6 (2017): 889–901. https://doi.org/10.1080/03639045.2017.1291672

10. Dhoot, A. S., G. J. Fernandes, A. Naha, et al. “Design of Experiments in Pharmaceutical Development.” Pharmaceutical Chemistry Journal 53 (2019): 730–35. https://doi.org/10.1007/s11094-019-02070-4

11. Arnold, S. F. “Design of Experiments with MINITAB.” The American Statistician 60, no. 2 (2006): 205. https://doi.org/10.1198/tas.2006.s46

12. Wahid, Z., and N. Nadir. “Improvement of One Factor at a Time through Design of Experiments.” World Applied Sciences Journal 21, no. 1 (2013): 56–61.

13. ElGamal, R., C. Song, A. M. Rayan, C. Liu, S. Al- Rejaie, and G. ElMasry. “Thermal Degradation of Bioactive Compounds during Drying Process of Horticultural and Agronomic Products: A Comprehensive Overview.” Agronomy 13, no. 6 (2023): 1580. https://doi.org/10.3390/agronomy13061580

14. Müller, J., A. Heindl. “Drying of Medicinal Plants.” Frontis (2006): 237–52.

15. Nair, G. R., P. Liplap, Y. Gariepy, and G. S. V. Raghavan. “Effect of Microwave and Hot Air Drying on Flax Straw at Controlled Temperatures.” International Journal of Postharvest Technology and Innovation 2, no. 4 (2012): 355– 69.

16. Nair, G. R., K. A. E. L. Eggie, Y. V. A. N. Gariépy, V. A. L. É. R. I. E. Orsat, and V. I. J. A. Y. A. Raghavan. “Microwave Assisted Drying of Flax Straw and Fibre at Controlled temperatures. In CSBEI00614 Presented at Section VI: Postharvest Technology and Process Engineering Conference.